OSMT (Ontario Society of Medical Technologists) Practice Exam

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What needs to be confirmed to ensure the quality of prepared media?

4% is tested
Biochemical tests are run with known organisms
Contaminant counts are determined
Plates are aerobically incubated at 22C

The correct answer is: Biochemical tests are run with known organisms

To ensure the quality of prepared media, it is essential to run biochemical tests with known organisms. This practice verifies that the media supports the growth of specific microorganisms and that it has the necessary nutritional components and environment required for those organisms. Performing these tests allows for the assessment of the media's effectiveness in supporting microbial growth and activity, ensuring that it is performing as expected. Conducting such tests helps confirm the media's suitability for diagnostic and research purposes, as different organisms have varying requirements in terms of pH, nutrients, and oxygen levels. By using known organisms, one can validate that the media's formulation is correct and that it will yield reliable results in practical applications. While other options may relate to aspects of media quality or testing, they do not directly confirm the media's capability to support the growth of target microorganisms in the same rigorous manner as running biochemical tests with known organisms.